Enzyme

Hello, class!

This week, I got an experiment about enzyme.

Enzymes are essential catalysts, which help to occur chemical reaction rapidly in our body.   Depends on the environmental conditions (PH and temperature) and activators will change their shape and alter its rate of activity.


The purpose of this experiment is to understand how does the enzyme work following experiments: investigating catechol oxidizes activity, the inhibition of enzyme activity, and the influence of factors (concentration, pH, and temperature) on the activity of the enzyme.


In our group, we were using 7 named test tubes, I put each 5mL of pH (4,5,6,6.8,7,8,9) buffer separately for each test tube. Then 1.5mL amylase was put into the test tubes by clean pipette and mixed them well. A drop of I2KI was put into the test plate and each test tube was recorded the time independently. Just before recording the time, 2.5mL of 1% starch solution was put into a test tube. Those procedure was rotated every test rube.

 It is very clear starch breakdown is most efficient at pH7 and gradually decrease as far from pH7. This is because Amylase is usually in the human mouth and exists with water which pH is 7.